Learning Objectives:

• Describe the structure of the Gram-positive and Gram-negative bacteria.
• Appreciate theoretical and technical aspects of the Gram staining procedure.
• Know the most commonly made mistakes in Gram staining.
• Critically interpret the results of a Gram staining experiment using a light microscope.

SOLUTION

In this lab, you will be using principles of Gram staining to identify a pathogen found in a cerebrospinal fluid (CSF) sample. A CSF sample is typically acquired using a lumbar puncture, often referred to as a spinal tap. A lumbar puncture is performed by inserting a needle between the L3 and L4 or L4 and L5 vertebra and extracting somewhere between 1 to 15 ml of CSF.……Please click the icon below to access entire solution at $10