Learning Objectives:

• Explain the principle of different ELISA techniques.
• Apply sandwich ELISA to quantify protein samples.
• Analyze the standard curve of ELISA experiment.
• Understand the function of reagents and equipment used in ELISA.
• Describe the basic troubleshooting process of ELISA.

SOLUTION

In this lab simulation, the term hapten is used to reference the size of molecules. A hapten is a small molecule that binds with a protein carrier to elicit antibody production. Within the lab, four types of ELISA testing are covered. The type of ELISA testing used will vary based on whether you are assessing antibody or antigen activity and whether you are measuring small or large molecules. There are conflicting definitions on what constitutes a small vs a large molecule.….Please click the icon below to access entire solution at $10